Saka returned from his hamstring injury with two substitute appearances against Fulham and Everton last week.
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Arsenal have no fresh injury concerns from their 1-1 draw at Everton on Saturday, with Mikel Merino fine after cutting his head to start up top.
Ben White was able to get through 60 minutes at Goodison Park, starting a game for the first time since November, but Jurrien Timber gets the nod a right-back with Jakub Kiwior alongside William Saliba at centre-back.
Declan Rice, Martin Odegaard and Thomas Partey are Arsenal’s midfield trio. Odegaard and Partey were both named on the bench at Everton. Raheem Sterling is suspended.
Arsenal XI: Arsenal: Raya, Timber, Saliba, Kiwior, Lewis-Skelly, Partey, Rice, Odegaard, Saka, Martinelli, Merino
Subs: Neto, Setford, Tierney, White, Zinchenko, Trossard, Jorginho, Butler-Oyedeji, Gower, Nwaneri
Real Madrid XI: Courtois; Valverde, Asencio, Rudiger, Alaba; Modric, Camavinga, Bellingham; Rodrygo, Mbappe, Vinicius Junior
Subs: Fran Gonzalez, Mestre, Guler, Enrick, Lucas Vazquez, Vallejo, Fran Garcia, Brahim, Gonzalo, Jacobo, Chema, Lorenzo
Time and date: 8pm BST on Tuesday, April 8, 2025
How to watch: Amazon Prime